50+ Microbiology MCQ FOR NURSING OFFICER

 Microbiology MCQ For Nursing Competition

Q.1 The Following Are The Types of Autoclave, Except ?

(A). Simple Iron Jacketed

(B). Low Pressure Low Temperature

(C). High Pressure High Vacuum

(D). High Pressure Low Vacuum

[ Correct Answer Is Option - D. ]

Q.2 Gloves Are Sterilized By Autoclaving For ?

(A). 20 Min. At 250 Degree F to 254 Degree F

(B). 30 Min. At 200 Degree F to 204 Degree F

(C). 20 Min. At 200 Degree F to 204 Degree F

(D). 30 Min. At 250 Degree F to 254 Degree F

[ Correct Answer Is Option - A. ]

[ Rationale - Sterilization Condition of Autoclave Are 121 Degree C (250 Degree F) Temperature, 15 Pound (LBS) Pressure And 15 To 20 Minute Holding Time (time is varies according material used).

It Was Invented By Charles Chamberland In 1884. ]

Q.3 What Is The Correct Temperature Used In Autoclave Method To Sterilize Instruments ?

(A). 40 Degree C For 10 Min.

(B). 100 Degree C For 25 Min.

(C). 126 Degree C For 20 Min.

(D). 110 Degree C For 15 Min.

[ Correct Answer Is Option - C. ]

Q.4 Time Required For Autoclaving At 15 LBS Per Square Inch Pressure And 121 Degree C Temperature Is ?

(A). 15 Min.

(B). 30 Min.

(C). 20 Min.

(D). 25 Min.

[ Correct Answer Is Option - A. ]

Q.5 The Best Method To Sterilize Instrument Is ?

(A). Place It In A Chemical Germicide

(B). Put In Into An Autoclave

(C). Boil It For Twenty Minutes

(D). Pass It Through A Bright Flame

[ Correct Answer Is Option - B. ]

[ Rationale :- Among These Autoclave Is The Best Method of Sterilization Because Steam Above 100 Degree C or Saturated Moist Heat Has A Better Killing And Penetration Power Than Dry Heat. ]

Q.6 The Pressure Set Into Autoclave For Sterilization Is ?

(A). 15 LBS

(B). 5 LBS

(C). 10 LBS

(D). 20 LBS

[ Correct Answer Is Option - A. ]

Q.7 Temperature Used Into Autoclave For Effective Sterilization ?

(A). 100 Degree C

(B). 110 Degree C

(C). 121 Degree C

(D). 131 Degree C

[ Correct Answer Is Option - C. ]

Q.8 Method of Sterilization For Articles Used Into Dressing ?

(A). Hot Air Oven

(B). Autoclave

(C). Filtration

(D). Chemical Sterilization 

[ Correct Answer Is Option - B. ]

Q.9 The Most Popular And Useful Method For Sterilization Is ?

(A). Boiling

(B). Autoclave

(C). Sunlight

(D). Ionizing Radiation

[ Correct Answer Is Option - B ]

Q.10 An Autoclave Is Used For ?

(A). Dry Heat Sterilization

(B). Moist Heat Sterilization

(C). Chemical Sterilization

(D). Gaseous Sterilization

[ Correct Answer Is Option - B. ]

[ Rationale :- Moist Heat Sterilization Performed At Different Temperature. Temperature Below 100 Degree C (ex. Pasteurization, Inspissations etc.). Temperature At 100 Degree C ( ex. Boiling, Tyndallisation etc.). Temperature Above 100 Degree C (ex. Autoclave).

Q.11 Which of Following Suture Materials Can Be Autoclaved For Sterilization ?

(A). Silk & Liner

(B). Catgut & Polyglactin

(C). PDS & Polyster

(D). Polyglycolic Acid & Collagen

[ Correct Answer Is Option - A. ]

Q.12 Which Method Is Not Use In Sterilization of Syringes And Needles ?

(A). Antiseptic Solutions

(B). Autoclaving

(C). Boiling

(D). Steam Sterilization

[ Correct Answer Is Option - A. ]

Q.13 Which of The Following Destroys HIV ?

(A). Savlon

(B). Sodium Hypochlorite

(C). Lysol

(D). Dettol

[ Correct Answer Is Option - B.]

[ Rationale :- Chlorine Compound Like Bleaching Powder (sodium hypochlorite) Are Most Effectively Used To Sterilize HIV Infected Material.

Glutaraldehyde Is Also A Sterilizing Agent Effective Against All Microorganisms Including Viruses (like HIV) And Spores.]

Q.14 The Solution Used To Disinfected The Bed Line of HIV Positive Patient Is ?

(A). Dettol

(B). Lysol

(C). Savlon

(D). Sodium Hypochlorite

[ Correct Answer Is Option - D ]

Q.15 HIV Contaminated Waste Products Can Be Decontaminated By The Following Agents  Except ?

(A). Sodium Hypochlorite

(B). Formaldehyde

(C). Glutaraldehyde

(D. Methanol

[ Correct Answer Is Option - D ]

Q.16 Which of The Following Is Used To Destroy HIV Contamination Caused By Body Fluids of HIV Patient ?

(A). Dettol

(B). Savlon

(C). Sodium Hypochlorite

(D). Lysol

[ Correct Answer Is Option - C ]

Q.17 To Make An Instrument Free of HIV Virus, It Is Kept In ?

(A). Spirit For 24 Hours

(B). 2% Glutaraldehyde Solution

(C). Savlon Solution (1:100 strength)

(D). Hydrogen Peroxide

[ Correct Answer Is Option - B ]

Q.18 Recommended Disinfectant Used For Handling Spills Is ?

(A). 10% Sodium Hypochlorite

(B). 1% Sodium Hypochlorite

(C). 5% Bleach Solution

(D). 2% Bleach Solution

[ Correct Answer Is Option - A ]

[ Rationale :- For Cleaning Up Spills of Blood Borne Pathogens Use Freshly Prepared 10% Solution of Household Bleach (One Part Bleach And 9 Part Water) Or Other Chlorine And Iodine Based Disinfectant.

Sodium Hypochlorite When Dissolve Into Water It Is Commonly Known As Bleach, Which Is Frequently Use As Disinfectant Or A Bleaching Agent For Household. ]

Q.19 Cresol, Lysol And Chloroxylenol Are ?

(A). Halogens

(B). Phenols

(C). Acids

(D). Aldehydes

[ Correct Answer Is Option - B ]

[ Rationale :- Cresol, Lysol (solution of cresols into soap called lysol), Chloroxylenol (active ingredient of dettol) And Chlorhexidine (chlorhexidine and cetrimide called savlon) Are Some Phenol Derivatives Used As Antiseptics. ]

Q.20 Which of The Following Chemical Is Most Powerful Disinfectant ?

(A). Dettol

(B). Phenol

(C). Lysol

(D). KMnO4

[ Correct Answer Is Option - B ]

[ Rationale :- Phenolic Is A Intermediate Level Disinfectants. Phenolic Disinfectants Are Effective Bactericides, Fungicides, Tuberculocides And Virucides, But Are Ineffective Against Spore Forming Bacteria.

Potassium Permanganate (KMnO4) Is A Common Inorganic Chemical Used To Treat Drinking Water For Iron, Manganese And Sulfur Odors.

It Can Be Used As A Disinfectant To Keeping Drinking Water Free of Harmful Bacteria. ]

Q.21 The Agent Which Kills Pathogenic Bacteria Is Called ?

(A). Asepsis

(B). Disinfectant

(C). Fomite 

(D). Bacteriostat

[ Correct Answer Is Option - B ]

[ Rationale :- Disinfectants Are Antimicrobial Agents That Are Applied To Nonliving Objects To Destroy Microorganism Present On Them.

Asepsis Is A Condition of Making Free From Germs, Infection And Any Form of Life Not An Agent That Kill Pathogens.

Bacteriostat Are The Substance That Inhibiting Or Retarding Bacterial Growth. ]

Q.22 An Agent That Kills Microorganism Is ?

(A). Antiseptic

(B). Bacteriostatic

(C). Dettol

(D). Bactericidal

[ Correct Answer Is Option - A ]

Q.23 A Chemical Agent That Inhibits Bacterial Growth Is Called ?

(A). Disinfectant

(B). Bacteriostat

(C). Germicide

(C). Sterilant

[ Correct Answer Is Option - B ]

Q.24 After Doing All Procedures The Instruments Are Sterilized, Dry Sterilization Is Used For ?

(A). Cutting Instruments And Glass Ware

(B). Gauze And Swabs

(C). Gloves And Syringes

(D). Bed Sheets And Linen

[ Correct Answer Is Option - A ]

[ Procedure Use For Sterilization By Dry Heat Are Red Heat, Flaming, Incineration And Hot Air Oven. Surgical Instrument (like scalpels, scissors and forceps) And Glass Ware Are Mainly Sterilized By Dry Heat. Gauze, Swabs, Gloves, Bed Sheets And Linen Will Not Be Sterilized By Dry Heat. ]

Q.25 Which of These Paint Is Applied On Skin As Antiseptic ?

(A). Gentian Violet Paint

(B). Mandl's Paint

(C). Glycerin Paint

(D). Burger Paint

[ Correct Answer Is Option - A ]

[ Rationale :- Gentian Violet Used For Dressing of Infected Wound And Painting of Body Cavities Infected With Fungus Like Vagina And Oral Cavity.

Mandl's Paint Is A Preparation of Iodine And Glycerine Used To Treat Server Throat Infection (pharyngitis of tonsillitis). ]

Q.26 Cotton For Dressing Is Sterilized By ?

(A). Autoclaving

(B). By Dipping In Water

(C). By Burning In Spirit

(D). None of These

[ Correct Answer Is Option - A ]

Q.27 Eusol Is Used For ?

(A). To Stop Cough

(B). To Prevent Jaundice

(C). Dressing

(D). To Dissolve Medicines

[ Correct Answer Is Option - C ]

[ Rationale :- Eusol Is An Antiseptic Solution Prepared From Chlorinated Lime Or Bleaching Powder (1.25gm) And Boric Acid (12.5 gm) Used For Cleaning of Wound. ]

Q.28 Which of Following Is Not A Chemical Disinfectant ?

(A). Glutaraldehyde

(B). Lysol

(C). Isopropyl Alcohol

(D). Ethylene Oxide

[ Correct Answer Is Option - D ]

[ Rationale :- Glutaraldehyde (commercial name cidex ), Lysol (phenol derivates) And Isopropyl Alcohol (isopropyl Alcohol And ethyl alcohol are most frequently used alcohol as a chemical disinfectant) Are Use As Chemical Disinfectant.

Ethylene Oxide Is A Colorless Gas Used For Sterilization of Heat & Moisture Sensitive Articles. ]

Q.29 Temperature For Autoclaving AFB Is ?

(A). 100 Degree C

(B). 110 Degree C

(C). 120 Degree C

(D). 150 Degree C

[ Correct Answer Is Option - C ]

Q.30 Red Heat Is Used For Sterilization of ?

(A). Inoculation Wires And Loops

(B). All Glass Syringes

(C). Soiled Dressings

(D). Glass Slides

[ Correct Answer Is Option - A ]

[ Rationale :- Red Heat Or Flaming (a method of dry heat sterilization) Is Used For Sterilization of Inoculating Wires Or Loops And Tips of Forceps & Needles. All Glass Syringes Or Slides (sterilize by hot air oven) And Soiled Dressings (sterilize by incineration) Are Not Sterilize By Use of Red Heat.

Q.31 Inoculating Loop Is Sterilized By ?

(A). Hot Air Oven

(B). Autoclave

(C). Flaming

(D). Radiation

[ Correct Answer Is Option - C ]

Q.32 Exposure of Material To Steam At 100 Degree C For 20 Minute On Three Consecutive Days Is Known As ?

(A). Tyndallisation

(B). Autoclaving

(C). Inspissation

(D). None of The Above

[ Correct Answer Is Option - A ]

[ Rationale - Exposure of Material To Steam At 100 Degree C For 20 Minutes On Three Consecutive Days Is Known As Tyndallisation Or Intermittent Sterilization.

It Only Destroys The Pathogen Into Vegetative From Not Into Spore.

It Is Used For Sterilization of Eggs, Serum And Sugar Containing Media. ]

Q.33 The Process By Which, Medium Is Placed At 100 Degree C In Flowing Steam For 30 Minutes Each On 3 Successive Days Is ?

(A). Steam Under Pressure

(B). Autoclave

(C). Tyndallization

(D). Inspissation

[ Correct Answer Is Option - C ]

Q.34 Natural Agent For Disinfection Is ?

(A). Burning

(B). Boiling

(C). Sunlight

(D). Radiation

[ Correct Answer Is Option - C ]

[ Rationale :- Sunlight Is A Natural Method of Sterilization Used For Sterilization of Water Into Tank, River And Lakes. Sunlight Contain Ultraviolet Rays Which Is Germicidal Into Nature.

Q.35 Best Method To Sterilise Catheters ?

(A). Fumigation

(B). Hot Air Sterilization

(C). By Ultra Violet Rays

(D). Boiling

[ Correct Answer Is Option - C ]

[ Rationale :- Non-Ionizing Radiations Like Ultraviolet Or Infrared Are Use To Sterilize Catheters And Syringes. Ionizing Radiations Like Gamma Rays Or X-Rays Are Use To Sterilize Disposable Items Such As Plastic Syringes, Swabs, Culture Plates, Cannulas And Catheters.

Q.36 The Best Method That Can Be Used To Sterilize A Catheter Is ? 

(A). Autoclave 

(B). Boiling

(C). Fumigation

(D). Radiation

[ Correct Answer Is Option - D ]

Q.37 To Sterilize Disposable Syringes, The Method of Sterilization Used Is ?

(A). Ethyloxide Autoclavation

(B). Infra Red Radiation

(C). Ultraviolet Radiation

(D). Gamma Radiation

[ Correct Answer Is Option - D ]

Q.38 The Gas Used For Fumigation of Operation Theaters And Wards Is ?

(A). Co2

(B). O2

(C). Formaldehyde

(D). Nitrogen

[ Correct Answer Is Option - C ]

[ Rationale :- The Disinfection of Rooms Or Operation Theatre By Gases Called Fumigation.

Formaldehyde (a colourless, pungent and irritant gas) Is Mainly Used For The Fumigation of Operation Theatre. Water Based Solution of Formaldehyde Called Formalin.

Ethylene Oxide Also Used For Fumigation And Gas Sterilization. ]

Q.39 Fumigation With Formalin Vapor Is Commonly Used To Sterilize And Disinfect ?

(A). Operation Room

(B). Scrubbing Room

(C). Nursing Room

(D). Sterilization Room

[ Correct Answer Is Option - A ]

Q.40 Among Which NOT Sterilize By Antiseptic Solution ?

(A). NG Tube

(B). ET Tube

(C). Scissors

(D). Endoscopes

[ Correct Answer Is Option - C ]

Q.41 Which of The Following Items Is Mot Sterilized By Antiseptic Solution ?

(A). Suction Catheter

(B). Enema Tube

(C). Surgical Scissor

(D). Nasogastric Tube

[ Correct Answer Is Option - C ]

Q.42 Endoscope Sterilize ?

(A). By 2% Phenol

(B). By 2% Glutaraldehyde 

(C). By UV Rays

(D). Autoclaving

[ Correct Answer Is Option - B ]

[ Rationale :- Endoscope, Bronchoscope And Cystoscope (An Instruments For Interior Examination of Bladder And Ureter) Are Sterilized By 2% Glutaraldehyde. ]

Q.43 Endoscopic Catheters Are Sterilized By ?

(A). Boiling For 10 Min

(B). Autoclaving

(C). 5% Savlon

(D). 2% Glutaraldehyde

[ Correct Answer Is Option - D ]

Q.44 Cross Infection Can Occur Through Following Excepts ?

(A). Fomites

(B). Autoclaving

(C). Contained Food And Drinks

(D). Insects

[ Correct Answer Is Option - B ]

[ Rationale :-  Cross Infection Means The Transfer of Infectious Organisms Or Disease From One Patient In A Hospital To Another. Means of Cross Infection Are Fomites, Intake of Contaminated Food And Drinks, Insects And Droplet Nuclei. Autoclaving Is A Technique of Reducing The Chance of Cross Infection. ]

Q.45 Heating In A Hot Oven at 160 Degree C For One Hour Is Used For Sterilization of ?

(A). All Glass Syringes

(B). Oils And Jellies

(C). Swab Sticks

(C). All of The Above

[ Correct Answer Is Option - A ]

[ Rationale :- Hot Air Oven Is A Widely Used Method of Sterilization By Dry Heat (temperature 160 Degree C for one to two hour). This Method Is Widely Used For Sterilization of Glass Wares (like glass syringes, Petri dishes, pipettes and test tubes) And Surgical Instrument (like scalpels, scissors and forceps). ]

Q.46 Glass Wares Are Sterilized By Hot Air Oven At ?

(A). 110 C For 1 Hour

(B). 120 C For 1 Hour

(C). 100 C For 1 Hour

(D). 160 C For 1 Hour

[ Correct Answer Is Option - D ]

Q.47 The Best Method of Sterilization of Syringe Is ?

(A). Autoclave

(B). Boiling

(C). Hot Air Oven

(D). Radiation 

[ Correct Answer Is Option - C ]

Q.48 The Best Method of Sterilization of Dressing Powder Is ?

(A). Autoclave

(B). Boiling

(C). Hot Air Oven

(D). Radiation

[ Correct Answer Is Option - C ]

Q.49 Items Not Be Placed Inside The Autoclave ?

(A). Glass

(B). Plastic

(C). Steel

(D). Aluminium

[ Correct Answer Is Option - A ]

Q.50 Disposable Syringes Are Sterilized By ?

(A). Boiling

(B). Ultraviolet Radiation

(C). Electromagnetic Radiation

(D). Ethylene Oxide

[ Correct Answer Is Option - C ]

[ Rationale :- Rays That Travel At The Speed of Light And Exhibit Both Magnetic And Electrical Properties Called Electromagnetic Radiation. Disposable Items X-Rays, Gamma Rays etc.

Q.51 Needles And Syringes Are Sterile Except ?

(A). By Antiseptic Solution

(B). By Hot Air Oven

(C). By Autoclaving

(D). By Boiling

[ Correct Answer Is Option - B ]

Q.52 Autoclaving A Means of Sterilization Will Destroy ?

(A). All Microorganisms

(B). Pathogenic Microorganisms

(C). Non-pathogenic Microorganisms

(D). Just Yeasts And Molds

[ Correct Answer Is Option - A ]

Q.53 Sharp Instruments Should Not Be Sterilized By ?

(A). Autoclaving

(B). Boiling

(C). Hot Air Oven

(D), Antiseptic Solution

[ Correct Answer Is Option - B ]

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